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KMID : 0381920070370020135
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Korean Journal of Microscopy
2007 Volume.37 No. 2 p.135 ~ p.142
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Morphological Study on the TUNEL Reaction of Mouse Ovary by X-ray Irradiation
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Yoon Chul-Ho
Chang Byung-Soo
Chang Byung-Soo
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Abstract
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This research investigated morphological changes of ovarian follicle according to dose of irradiation when
adult mice were exposed to X-rays from 6 MeV LINAC. At day 3 after irradiation of 200 cGy, 400 cGy and
600 cGy X-ray to the hole body of mice, the ovaries collected and stained with TUNEL. The normal follicles
and atretic follicles were identified to apoptosis by the staining with TUNEL. In the atretic follicles of the normal
ovary, the apoptotic bodies were well appeared and stained brown color. Almost of the follicles following
irradiation are stained with TUNEL, but the sensitivity of reaction is weaker than that in irradiation of 400 cGy
and 600 cGy X-ray. The granulosa cells of the radiated normal follicle by 400 cGy are shown brown color. In
this stage, the nucleus of granulosa cells in the atrectic follicles are condensed and picknotic feature. The size
of the radiated follicle by 600 cGy are decreased than the normal follicles. The atropic follicles are filled with
apoptotic bodies which change of granulosa cells and theca cells by influence of X-ray. All of cell in the follicles
are strongly positive stained with TUNEL by irradiation of 600 cGy.
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KEYWORD
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Apoptosis, Granulosa cell, Ovarian follicle, Mouse, X-ray
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